GNI4310-GP/Anti-GFP-HRP

GNI集团抗体GNI4310-GPAnti-GFP-HRP ，本实验使用不同稀释比例的Anti-GFP (8C1)单克隆抗体（HRP偶联）对表达GFP标签蛋白的大肠杆菌提取物进行Western Blot分析，以评估抗体的最佳工作浓度。抗体特异性针对天然和变性的GFP或GFP融合蛋白，适用于多种实验应用（如WB、Dot、ELISA）。实验结果显示，抗体在1:2000至1:8000的稀释范围内均能有效检测目标蛋白。

GNI4310-GPAnti-GFP-HRP

项目	信息	Information
产品名称	Anti-GFP-HRP	Anti-GFP-HRP
特异性	天然和变性的GFP或所有物种中的GFP融合蛋白	Native and denatured forms of GFP or GFP fusion protein in all species
应用	WB: 1:500-1:5000 Dot: 1:500-1:5000 ELISA: 1:500-1:5000	WB: 1:500-1:5000 Dot: 1:500-1:5000 ELISA: 1:500-1:5000
偶联物	HRP	HRP
靶标	GFP	GFP
免疫原	合成的GFP标签肽与KLH偶联	Synthetic GFP tag peptide coupled to KLH
表位	CMSKGEELFTGVVPILVELDG	CMSKGEELFTGVVPILVELDG
宿主物种	小鼠	Mouse
克隆性	单克隆	Monoclonal
克隆号	8C1	8C1
同种型	IgG1	IgG1
形式	液体	Liquid
储存缓冲液	50%甘油，150mM氯化钠，10mM磷酸钠，pH 7.4	50% Glycerol, 150mM Sodium Chloride, 10mM Sodium Phosphate, pH 7.4
浓度	0.5 mg/mL	0.5 mg/mL
运输和储存	4°C运输，-20°C储存，避免反复冻融	Ship at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

Western Blot分析不同浓度的Anti-GFP (8C1)单克隆抗体

泳道号	稀释比例	抗原
1	1:2000	表达GFP标签蛋白的大肠杆菌提取物
2	1:4000	表达GFP标签蛋白的大肠杆菌提取物
3	1:8000	表达GFP标签蛋白的大肠杆菌提取物

Western Blot Analysis of Different Concentrations of Anti-GFP (8C1) Monoclonal Antibody

Line No.	Dilution	Antigen
1	1:2000	Extracts from E.coli expressing GFP-tagged protein
2	1:4000	Extracts from E.coli expressing GFP-tagged protein
3	1:8000	Extracts from E.coli expressing GFP-tagged protein

GNI4310-GP/Anti-GFP-HRP(图1)

Western blot analysis of different concentration of Anti-GFP (8C1) Monoclonal Antibody

Antigen: Extracts from E.coli expressing GFP-tagged protein

Line No.1: 1:20002: 1:40003: 1:8000

Product Name	Anti-GFP-HRP
Specificity	Native and denatured forms of GFP or GFP fusion protein in all species
Applications	WB: 1:500-1:5000 Dot: 1:500-1:5000 ELISA: 1:500-1:5000
Conjugated	HRP
Target	GFP
Immunogen	Synthetic GFP tag peptide coupled to KLH
Epitope	CMSKGEELFTGVVPILVELDG
Host Species	Mouse
Clonality	Monoclonal
Clone No.	8C1
Isotype	IgG1
Form	Liquid
Storage Buffer	50% Glycerol, 150mM Sodium Chloride, 10mM Sodium Phosphate, pH 7.4
Concentration	0.5 mg/mL
Transportation and Storage	Ship at 4°C. Store at -20°C. Avoid freeze / thaw cycle.