GNI4510-V5/ Anti-V5 Affinity Gel
GNI4510-V5/Anti-V5 Affinity Gel ,本品为小鼠单克隆抗体（，同种型IgG1）偶联Sepharose 4B的亲和填料，特异性结合V5标签蛋白（表位：GKPIPNPLLGLDST）。适用于重组蛋白纯化和免疫沉淀（IP）实验，具有高亲和力和批次间一致性，可高效捕获和洗脱V5标签融合蛋白。实验示例显示其对大肠杆菌表达系统的V5标签蛋白具有显著富集效果。
GNI4510-V5/Anti-V5 Affinity Gel ,本品为小鼠单克隆抗体（，同种型IgG1）偶联Sepharose 4B的亲和填料，特异性结合V5标签蛋白（表位：GKPIPNPLLGLDST）。适用于重组蛋白纯化和免疫沉淀（IP）实验，具有高亲和力和批次间一致性，可高效捕获和洗脱V5标签融合蛋白。实验示例显示其对大肠杆菌表达系统的V5标签蛋白具有显著富集效果。
GNI4510-V5/Anti-V5 Affinity Gel
产品概述 (Product Overview)
本品为小鼠单克隆抗体（，同种型IgG1）偶联Sepharose 4B的亲和填料，特异性结合V5标签蛋白（表位：GKPIPNPLLGLDST）。适用于重组蛋白纯化和免疫沉淀（IP）实验，具有高亲和力和批次间一致性，可高效捕获和洗脱V5标签融合蛋白。实验示例显示其对大肠杆菌表达系统的V5标签蛋白具有显著富集效果。
| 产品名称 (Product Name) | Anti-V5 Affinity Gel |
| 特异性 (Specificity) | 所有物种中的V5标签蛋白 (V5-tagged protein in all species) |
| 应用 (Applications) | 重组蛋白纯化 (rProtein Purification): 每毫升填料至少洗脱0.5 mg融合蛋白 免疫沉淀 (IP): 5 µL用于500 µL粗蛋白溶液 |
| 偶联物 (Conjugated) | Sepharose 4B |
| 靶点 (Target) | V5标签 (V5 tag) |
| 免疫原 (Immunogen) | 合成的V5标签肽段偶联KLH (Synthetic V5 tag peptide coupled to KLH) |
| 表位 (Epitope) | GKPIPNPLLGLDST |
| 宿主物种 (Host Species) | 小鼠 (Mouse) |
| 克隆性 (Clonality) | 单克隆 (Monoclonal) |
| 克隆号 (Clone No.) | 10B5 |
| 同种型 (Isotype) | IgG1 |
| 储存缓冲液 (Storage Buffer) | 50%甘油, 150mM氯化钠, 10mM磷酸钠, pH 7.4, 含0.02% (w/v) 叠氮化钠 |
| 浓度 (Concentration) | 50%凝胶悬浮于储存缓冲液中 (50% gel suspension in storage buffer) |
| 运输和储存 (Transportation and Storage) | 运输温度：4°C 储存温度：-20°C 避免反复冻融 (Avoid freeze / thaw cycle) |
| 免疫沉淀实验 (Immunoprecipitation) | 样品：表达V5标签蛋白的大肠杆菌提取物 (Sample: extracts from E.coli expressing V5-tagged protein) |
| 1: SGT Anti-V5 Affinity Gel与大肠杆菌周质提取物孵育 (SGT Anti-V5 Affinity Gel incubated with E.coli periplasmic extracts) | |
| 2: SGT Anti-V5 Affinity Gel阴性对照 (SGT Anti-V5 Affinity Gel negative control) |
Immunoprecipitation of anti-V5 affinity gel
Sample: extracts from E.coli expressing V5-tagged protein
Line No.
1: SGT Anti-V5 Affinity Gel incubated with E .coli periplasmic extracts
2: SGT Anti-V5 Affinity Gel negative control
| Product Name | Anti-V5 Affinity Gel |
| Specificity | V5-tagged protein in all species |
| Applications | rProtein Purification: minimum 0.5 mg fusion protein eluted per mL packed resin IP: 5 uL for 500 uL crude protein solution |
| Conjugated | Sepharose 4B |
| Target | V5 |
| Immunogen | Synthetic V5 tag peptide coupled to KLH |
| Epitope | GKPIPNPLLGLDST |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Clone No. | 10B5 |
| Isotype | IgG1 |
| Storage Buffer | 50% Glycerol, 150mM Sodium Chloride, 10mM Sodium Phosphate, pH 7.4, containing 0.02% (w/v) sodium azide |
| Concentration | 50% gel suspension in storage buffer |
| Transportation and Storage | Ship at 4°C. Store at -20°C. Avoid freeze / thaw cycle. |
上海睿远生物医药科技有限公司
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