GNI4510-V5/ Anti-V5 Affinity Gel

特异性 (Specificity)

所有物种中的V5标签蛋白 (V5-tagged protein in all species)

应用 (Applications)

重组蛋白纯化 (rProtein Purification): 每毫升填料至少洗脱0.5 mg融合蛋白
免疫沉淀 (IP): 5 µL用于500 µL粗蛋白溶液

偶联物 (Conjugated)

Sepharose 4B

靶点 (Target)

V5标签 (V5 tag)

免疫原 (Immunogen)

合成的V5标签肽段偶联KLH (Synthetic V5 tag peptide coupled to KLH)

表位 (Epitope)

GKPIPNPLLGLDST

宿主物种 (Host Species)

小鼠 (Mouse)

克隆性 (Clonality)

单克隆 (Monoclonal)

克隆号 (Clone No.)

10B5

同种型 (Isotype)

IgG1

储存缓冲液 (Storage Buffer)

50%甘油, 150mM氯化钠, 10mM磷酸钠, pH 7.4, 含0.02% (w/v) 叠氮化钠

浓度 (Concentration)

50%凝胶悬浮于储存缓冲液中 (50% gel suspension in storage buffer)

运输和储存 (Transportation and Storage)

运输温度：4°C
储存温度：-20°C
避免反复冻融 (Avoid freeze / thaw cycle)

免疫沉淀实验 (Immunoprecipitation)

样品：表达V5标签蛋白的大肠杆菌提取物 (Sample: extracts from E.coli expressing V5-tagged protein)

1: SGT Anti-V5 Affinity Gel与大肠杆菌周质提取物孵育 (SGT Anti-V5 Affinity Gel incubated with E.coli periplasmic extracts)

2: SGT Anti-V5 Affinity Gel阴性对照 (SGT Anti-V5 Affinity Gel negative control)

Specificity

V5-tagged protein in all species

Applications

rProtein Purification: minimum 0.5 mg fusion protein eluted per mL packed resin

IP: 5 uL for 500 uL crude protein solution

Conjugated

Sepharose 4B

Target

V5

Immunogen

Synthetic V5 tag peptide coupled to KLH

Epitope

GKPIPNPLLGLDST

Host Species

Mouse

Clonality

Monoclonal

Clone No.

10B5

Isotype

IgG1

Storage Buffer

50% Glycerol, 150mM Sodium Chloride, 10mM Sodium Phosphate, pH 7.4, containing 0.02% (w/v) sodium azide

Concentration

50% gel suspension in storage buffer

Transportation and Storage

Ship at 4°C. Store at -20°C. Avoid freeze / thaw cycle.