货号：0407-25 Cadherin-1 或上皮钙黏蛋白 (E-cadherin) 是一种由 CDH1 基因编码的蛋白质。其突变与胃癌、乳腺癌、结直肠癌、甲状腺癌和卵巢癌相关。CDH1 也被指定为 CD324（分化簇 324），是一种肿瘤抑制基因。Cadherin-1 是钙黏蛋白超家族的经典成员，其编码的蛋白质是一种钙依赖性细胞间黏附糖蛋白，由五个细胞外钙黏蛋白重复序列、一个跨膜区域和一个高度保守的胞质尾部组成。该基因的突变与多种癌症相关，其功能丧失被认为通过增加增殖、侵袭和/或转移促进癌症进展。该蛋白质的胞外域介导细菌对哺乳动物细胞的黏附，而胞质域是内化所必需的。E-cadherin（上皮型）是钙黏蛋白家族中研究最深入的成员，其胞外域包含五个钙黏蛋白重复序列（EC1 ~ EC5）、一个跨膜域和一个与 p120-连环蛋白和 β-连环蛋白结合的胞内域。胞内域包含一个高度磷酸化区域，对 β-连环蛋白结合至关重要，因此对 E-cadherin 功能至关重要。
如有找不到的抗体产品，欢迎直接联系客服咨询！
| 产品名称 | E Cadherin Rabbit Polyclonal Antibody |
| E Cadherin 兔多克隆抗体 | |
| 抗体类型 | Rabbit Polyclonal Antibody |
| 兔多克隆抗体 | |
| 免疫原 | Synthetic peptide within mouse E-Cadherin aa 595-644 / 882 |
| 小鼠 E-Cadherin 氨基酸 595-644 / 882 的合成肽 | |
| 种属反应性 | Human, Mouse |
| 人类、小鼠 | |
| 验证应用 | WB, IF-Cell, FC |
| 蛋白质印迹 (WB)、细胞免疫荧光 (IF-Cell)、流式细胞术 (FC) | |
| 分子量 | Predicted band size: 97 kDa |
| 预测条带大小：97 kDa | |
| 阳性对照 | HCT116, SKOV-3, Hela |
| HCT116、SKOV-3、Hela | |
| 偶联 | Unconjugated |
| 非偶联 | |
| RRID | AB_3068640 |
| AB_3068640 | |
| 产品特性 | |
| - 形态 | Liquid |
| 液体 | |
| - 浓度 | 1ug/ul |
| 1ug/ul | |
| - 存放说明 | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| 解冻后储存于 +4℃。分装后储存于 -20℃。避免反复冻融。 | |
| - 存储缓冲液 | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| 1*PBS (pH7.4), 0.2% BSA, 40% 甘油。防腐剂：0.05% 叠氮化钠。 | |
| - 亚型 | IgG |
| IgG | |
| - 纯化方式 | Immunogen affinity purified |
| 免疫原亲和纯化 | |
| 应用稀释度 | |
| - WB | 1:500 |
| 1:500 | |
| - IF-Cell | 1:50-1:200 |
| 1:50-1:200 | |
| - FC | 1:50-1:100 |
| 1:50-1:100 | |
| 靶点 | |
| - 功能 | Cadherin-1 or Epithelial cadherin (E-cadherin), (not to be confused with the APC/C activator protein CDH1) is a protein that in humans is encoded by the CDH1 gene. Mutations are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. CDH1 has also been designated as CD324 (cluster of differentiation 324). It is a tumor suppressor gene. Cadherin-1 is a classical member of the cadherin superfamily. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells, and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. |
| Cadherin-1 或上皮钙黏蛋白 (E-cadherin) 是一种由 CDH1 基因编码的蛋白质。其突变与胃癌、乳腺癌、结直肠癌、甲状腺癌和卵巢癌相关。CDH1 也被指定为 CD324（分化簇 324），是一种肿瘤抑制基因。Cadherin-1 是钙黏蛋白超家族的经典成员，其编码的蛋白质是一种钙依赖性细胞间黏附糖蛋白，由五个细胞外钙黏蛋白重复序列、一个跨膜区域和一个高度保守的胞质尾部组成。该基因的突变与多种癌症相关，其功能丧失被认为通过增加增殖、侵袭和/或转移促进癌症进展。该蛋白质的胞外域介导细菌对哺乳动物细胞的黏附，而胞质域是内化所必需的。E-cadherin（上皮型）是钙黏蛋白家族中研究最深入的成员，其胞外域包含五个钙黏蛋白重复序列（EC1 ~ EC5）、一个跨膜域和一个与 p120-连环蛋白和 β-连环蛋白结合的胞内域。胞内域包含一个高度磷酸化区域，对 β-连环蛋白结合至关重要，因此对 E-cadherin 功能至关重要。 | |
| 背景文献 | 1. Meigs T.E 等. J Biol Chem 277:24594-24600 (2002) 2. Agiostratidou G 等. J Neurochem 96:1182-1188 (2006) |
| 1. Meigs T.E 等. J Biol Chem 277:24594-24600 (2002) 2. Agiostratidou G 等. J Neurochem 96:1182-1188 (2006) | |
| 组织特异性 | Non-neural epithelial tissues |
| 非神经上皮组织 | |
| 翻译后修饰 | During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation (By similarity).; N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location.; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.; O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Thr-285 and Thr-509 are O-mannosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3. |
| 在细胞凋亡或钙离子内流时，被膜结合金属蛋白酶 (ADAM10)、PS1/γ-分泌酶和 caspase-3 切割。金属蛋白酶的切割导致细胞间黏附破坏，随后 β-连环蛋白释放到细胞质中。残留在膜上的切割产物通过细胞内蛋白水解途径迅速降解。caspase-3 的切割释放胞质尾部，导致肌动蛋白微丝系统解体。γ-分泌酶介导的切割促进黏附连接的解体。在耳蜗 Corti 器官发育过程中，ADAM10 在黏附连接处的切割促进支柱细胞分离（相似性）。Asn-637 的 N-糖基化对表达、折叠和运输至关重要。MGAT3 添加的双分 N-乙酰葡萄糖胺调节其细胞膜定位。含有 SKP2 的 SCF 复合物泛素化，需要 CK1/CSNK1A1 事先磷酸化。CBLL1/HAKAI 泛素化，需要在 Tyr-754 处事先磷酸化。O-糖基化。TMTC1、TMTC2、TMTC3 或 TMTC4 进行 O-甘露糖基化。Thr-285 和 Thr-509 由 TMTC2 或 TMTC4 进行 O-甘露糖基化，但不由 TMTC1 或 TMTC3 进行。 | |
| 亚细胞定位 | Cell membrane |
| 细胞膜 | |
| UNIPROT # | SwissProt: P12830 Human SwissProt: P09803 Mouse |
| SwissProt: P12830 人类 SwissProt: P09803 小鼠 | |
| 别名 | Arc 1 antibody, CADH1_HUMAN antibody, Cadherin 1 antibody, cadherin 1 type 1 E-cadherin antibody, Cadherin1 antibody, CAM 120/80 antibody, CD 324 antibody, CD324 antibody, CD324 antigen antibody, cdh1 antibody |
| Arc 1 抗体, CADH1_HUMAN 抗体, Cadherin 1 抗体, cadherin 1 type 1 E-cadherin 抗体, Cadherin1 抗体, CAM 120/80 抗体, CD 324 抗体, CD324 抗体, CD324 抗原抗体, cdh1 抗体 |
上海睿远生物医药科技有限公司
官方网址：www.fulaibio.com
Email: office01@fulaibio.com
电话：19946275258/18317065317
